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What is required during sterilization to achieve effective results?

  1. Short exposure to chemicals

  2. Holding at 250°F for fifteen minutes

  3. Cleaning with soap and water

  4. Applying a disinfecting agent

The correct answer is: Holding at 250°F for fifteen minutes

To achieve effective sterilization results, it is crucial to hold the instruments at a temperature of 250°F for fifteen minutes. This process is typically associated with autoclaving, which uses steam under pressure to eliminate all forms of microbial life, including bacteria, viruses, and spores. The specific temperature and time combination ensures that the sterilization process is thorough. At 250°F, the steam penetrates the materials and effectively destroys pathogens. It is important to maintain this temperature for a sufficient duration to guarantee that all microorganisms are eradicated, providing assurance that the tools are safe for use. Other methods mentioned, such as short exposure to chemicals, merely disinfect surfaces rather than sterilize, and cleaning with soap and water is primarily a preparatory step but not sufficient on its own for sterilization. Similarly, applying a disinfecting agent may reduce the number of bacteria but does not ensure complete sterility. Thus, the specified temperature and time for sterilization is a proven method to secure the highest level of infection control in a tattoo practice.